Complementary Solutions for IHC
FOR YOUR LAB: DETECTION SYSTEM ESSENTIALS
The complexities facing research labs today require a wide selection of visualization and evaluation tools. Scientists must rely on robust, flexible and efficient immunohistochemistry applications as the cornerstone of their research toolkits.
Immunohistochemistry
Immunohistochemistry or IHC refers to the process of localizing proteins in cells of a tissue section exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis). IHC is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.
Essential Detection System Requirements:
- Consistent staining and easy visualization
- Specialized for the detection of antigens present in low concentrations
- Higher sensitivity and antibody efficiency
- High signal-to-noise ratio
- Optimized with both concentrated and pre-diluted antibodies
- Biotin-free designs eliminate endogenous biotin interference
Detection kits are used to visualize an antibody-antigen interaction and should enable access to difficult targets. Visualization of a target can occur in a number of ways. Most commonly, an antibody is conjugated to an enzyme, such as peroxidase, which will catalyze a color-producing reaction. The resulting chromogenic reaction helps visualize the location of the antigen, antibody and enzyme complex. Antibodies can also be tagged to a fluorophore, such as fluorescein or Alexa Fluors.
A Biotin-Free Example: Detect the Difference
“REVEAL” SPRING’S NOVEL IHC DETECTION SYSTEM
Other polymers on the market tend to bind to themselves, creating a large molecule that may limit functionality and sensitivity. Spring's REVEAL detection technology uses novel chemistry to deliver outstanding sensitivity and a high signal-to-noise ratio. Advantages include:
- Reveal Detection - Biotin Free
Biotin-free configuration, removes background associated with endogenous biotin - Non Polymer
The lack of a backbone creates a small molecule 1/8 the size of polymer molecules removing any steric hindrance associated with large polymer molecules - Unique Molecular Chemistry
Combining novel combinations of bio-conjugation and organic chemistry
Redefining Sensitivity and Specificity
HRP Detection:
REVEAL Polyvalent HRP-DAB Detection System
The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a secondary antibody- HRP conjugate which reacts with the primary antibody, and substrate-chromogen (DAB).
AEC Detection:
REVEAL Biotin-Free Polyvalent HRP AEC
The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a secondary antibody- HRP conjugate which reacts with the primary antibody, and substrate-chromogen (AEC).